{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:biomolecular-engineering-directed-enzyme-evolution","canonicalPath":"/knowledge/biomolecular-engineering/concepts/biomolecular-engineering-directed-enzyme-evolution","contentHash":"sha256:ccde98d572113c6a4d3d584cf13cf691d5124cfe293433aadfc2323a2f6abfd3","generatedAt":"2026-08-30T16:03:26.776Z","publicationDecision":{"recordId":"urn:maha:record:biomolecular-engineering-directed-enzyme-evolution","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:biomolecular-engineering-directed-enzyme-evolution","scope":"Limited to PACE architecture, selection coupling, mutation, experiments, and Methods. in “A system for the continuous directed evolution of biomolecules”; this candidate records the concept boundary and does not pool results from uncited systems or studies.","boundary":"Directed enzyme evolution does not by itself establish system-level performance, safety, manufacturability, scalability, economic advantage, clinical benefit, or deployment readiness.","claimKind":"theoretical-model","sourceIds":["source-biomolecular-engineering-pace"],"statement":"The cited source supports treating directed enzyme evolution as a distinct concept within the stated biomolecular engineering scope.","replication":{"asOfDate":"2026-08-24","assessment":"Independent replication and cross-platform transfer have not been compiled for this candidate; the evidence maturity refers only to the bounded source contract.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"No cross-source quantitative interval is asserted. Definitions, operating conditions, samples, instruments, and outcome measures must be checked against the exact cited locator during review."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-biomolecular-engineering-pace","url":"https://doi.org/10.1038/nature09929","title":"A system for the continuous directed evolution of biomolecules","rights":{"note":"The candidate uses original boundary language and a short paraphrase linked to the cited source. No source passage, figure, or table is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Kevin M. Esvelt","Jacob C. Carlson","David R. Liu"],"boundary":"A selection system does not guarantee access to every fitness landscape or safe use outside its experimental containment.","publisher":"Nature","establishes":"The paper introduces phage-assisted continuous evolution and demonstrates specified selection-coupled evolution experiments.","identifiers":[{"value":"10.1038/nature09929","scheme":"doi"}],"publishedAt":"2011-04-10","exactLocator":"PACE architecture, selection coupling, mutation, experiments, and Methods."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_cb072eb851c44303bc8c1e2edd83f422","rationale":"Directed enzyme evolution binds claim urn:maha:claim:biomolecular-engineering-directed-enzyme-evolution only to A system for the continuous directed evolution of biomolecules at PMC3084352 / NIHMS272507, PACE architecture, selection coupling, mutagenesis, T7 RNA-polymerase experiments, and Methods (full manuscript). The audit inspected accepted-manuscript at full-document depth and recorded subject and claim support; the claim remains limited to “Limited to PACE architecture, selection coupling, mutation, experiments, and Methods. in “A system for the continuous directed evolution of biomolecules”; this candidate records the concept boundary and does not pool results from uncited systems or studies.”. This decision applies only to record urn:maha:record:biomolecular-engineering-directed-enzyme-evolution at sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:09.026Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_259e667e4dd44b4191de6cb910d7427f","rationale":"Directed enzyme evolution remains within domain biomolecular-engineering. Its mechanism or method is the bounded proposition “The cited source supports treating directed enzyme evolution as a distinct concept within the stated biomolecular engineering scope.”; the record does not transfer that proposition beyond Directed enzyme evolution does not by itself establish system-level performance, safety, manufacturability, scalability, economic advantage, clinical benefit, or deployment readiness. This decision applies only to record urn:maha:record:biomolecular-engineering-directed-enzyme-evolution at sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:09.094Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_d509761462444196a868d609614f61d8","rationale":"Directed enzyme evolution retains uncertainty “No cross-source quantitative interval is asserted. Definitions, operating conditions, samples, instruments, and outcome measures must be checked against the exact cited locator during review.” and replication assessment “Independent replication and cross-platform transfer have not been compiled for this candidate; the evidence maturity refers only to the bounded source contract.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:biomolecular-engineering-directed-enzyme-evolution at sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:09.197Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"rights-and-locator","verdict":"approve","reviewId":"epireview_71c23cf5bcd64aa68426e67e0cd00c3f","rationale":"A system for the continuous directed evolution of biomolecules is identified by doi:10.1038/nature09929, inspected at PACE architecture, selection coupling, mutation, experiments, and Methods., and retained under citation-with-paraphrase. This approval binds only exact revision sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd. This decision applies only to record urn:maha:record:biomolecular-engineering-directed-enzyme-evolution at sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:09.267Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:84b5247c53d2e106390e04dacfd9325874769cf98670ad9f272a53d176b574cd","supersedesReviewId":null,"reviewerProfileVersion":1}]}