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Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-lazzarotto-change-seq-2020","url":"https://www.nature.com/articles/s41587-020-0555-7","title":"CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Christopher R. Lazzarotto","Nhu T. Nguyen","J. A. Tangprasertchai","S. C. Malagon-Lopez","et al."],"boundary":"In-vitro nomination is not identical to editing frequency or biological consequence in a treated cell population or organism.","publisher":"Nature Biotechnology","establishes":"The study develops an in-vitro circularized-DNA assay for Cas9 activity and compares nominations with cellular activity and genomic context for specified targets.","identifiers":[{"value":"10.1038/s41587-020-0555-7","scheme":"doi"}],"publishedAt":"2020-06-22","exactLocator":"Abstract; Figures 1–6; Methods; datasets PRJNA625995 and GSE149295.","conflictsOfInterest":"The article declares patent and company relationships involving genome-editing assays and therapeutics."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_0eb5c7ad76674d83b817f2c4a3a41b3c","rationale":"Cell-line versus primary-cell evidence binds claim urn:maha:claim:cell-line-versus-primary-cell-evidence only to CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity at PMC7652380 author manuscript, “Chromatin state influences genome editing activity” and “Human genetic variation impacts genome editing nuclease activity”. The audit inspected repository-copy at bounded inspected artifact depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity.”. This decision applies only to record urn:maha:record:cell-line-versus-primary-cell-evidence at sha256:6b6b99f6540b8e46e2895fcacf978dc6cea7ca8688fe377cbce0201b6957b54d and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:03.350Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:cell-line-versus-primary-cell-evidence at sha256:6b6b99f6540b8e46e2895fcacf978dc6cea7ca8688fe377cbce0201b6957b54d and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:03.467Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:cell-line-versus-primary-cell-evidence at sha256:6b6b99f6540b8e46e2895fcacf978dc6cea7ca8688fe377cbce0201b6957b54d and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:03.530Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:cell-line-versus-primary-cell-evidence at sha256:6b6b99f6540b8e46e2895fcacf978dc6cea7ca8688fe377cbce0201b6957b54d and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:03.603Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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