{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:somatic-versus-germline-editing","canonicalPath":"/knowledge/synthetic-biology/comparisons/somatic-versus-germline-editing","contentHash":"sha256:93822d3f525b540db478076159aa36c1e561ef78b3dba595b81a9ada824258ee","generatedAt":"2026-08-30T16:03:12.639Z","publicationDecision":{"recordId":"urn:maha:record:somatic-versus-germline-editing","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:somatic-versus-germline-editing","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes.","boundary":"The experiment does not establish safe or acceptable reproductive use; germline questions require distinct ethical, legal, clinical, and multigenerational evidence.","claimKind":"empirical-claim","sourceIds":["source-liang-embryo-editing-2015"],"statement":"The cited embryo study reports mosaic and unintended outcomes after CRISPR-Cas9 exposure of non-viable tripronuclear human zygotes.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-liang-embryo-editing-2015","url":"https://link.springer.com/article/10.1007/s13238-015-0153-5","title":"CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Puping Liang","Yanwen Xu","Xiya Zhang","Chenhui Ding","et al."],"boundary":"The experiment does not establish reproductive safety, heritable clinical benefit, ethical acceptability, or authorization for germline use.","publisher":"Protein & Cell","establishes":"The study reports CRISPR-Cas9 editing outcomes, mosaicism, and unintended events in non-viable tripronuclear human zygotes under the stated protocol.","identifiers":[{"value":"10.1007/s13238-015-0153-5","scheme":"doi"}],"publishedAt":"2015-04-18","exactLocator":"Abstract; Results; Tables 1–2; Methods; ethical statement."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_25da8bc7b2af4ccba158a58251da2b11","rationale":"Somatic versus germline genome editing binds claim urn:maha:claim:somatic-versus-germline-editing only to CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes at Protein & Cell DOI 10.1007/s13238-015-0153-5, Abstract; HBB-editing Results; Discussion. The audit inspected version-of-record at declared inspected location depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes.”. This decision applies only to record urn:maha:record:somatic-versus-germline-editing at sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:06.136Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_f190a9513f8e44afb8c4ae6cb67207b3","rationale":"Somatic versus germline genome editing remains within domain synthetic-biology. Its mechanism or method is the bounded proposition “The cited embryo study reports mosaic and unintended outcomes after CRISPR-Cas9 exposure of non-viable tripronuclear human zygotes.”; the record does not transfer that proposition beyond The experiment does not establish safe or acceptable reproductive use; germline questions require distinct ethical, legal, clinical, and multigenerational evidence. This decision applies only to record urn:maha:record:somatic-versus-germline-editing at sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:06.211Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_a8c3b97a4cbe45f594c0412339c7e402","rationale":"Somatic versus germline genome editing retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:somatic-versus-germline-editing at sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:06.301Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"rights-and-locator","verdict":"approve","reviewId":"epireview_9b36676197f34cd58eb2a4e99601ff60","rationale":"CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes is identified by doi:10.1007/s13238-015-0153-5, inspected at Abstract; Results; Tables 1–2; Methods; ethical statement., and retained under citation-with-paraphrase. This approval binds only exact revision sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34. This decision applies only to record urn:maha:record:somatic-versus-germline-editing at sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:06.390Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:a70486150e1b435628d254e6e59be26d1e9e4f14c335458ec2f548af86d02f34","supersedesReviewId":null,"reviewerProfileVersion":1}]}