{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:genetic-toggle-switch","canonicalPath":"/knowledge/synthetic-biology/concepts/genetic-toggle-switch","contentHash":"sha256:3ec5a44f869becdddcb4c3fe159c4090a18d83b01af48c5ded5fee13149394d1","generatedAt":"2026-08-30T16:03:05.746Z","publicationDecision":{"recordId":"urn:maha:record:genetic-toggle-switch","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:genetic-toggle-switch","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Construction of a genetic toggle switch in Escherichia coli.","boundary":"The circuit’s memory and stability cannot be transferred to other hosts, copy numbers, growth conditions, burdens, or timescales.","claimKind":"empirical-claim","sourceIds":["source-gardner-toggle-2000"],"statement":"The cited study constructs a bistable toggle in Escherichia coli and reports switching thresholds and state behavior under defined induction conditions.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-gardner-toggle-2000","url":"https://www.nature.com/articles/35002131","title":"Construction of a genetic toggle switch in Escherichia coli","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Timothy S. Gardner","Charles R. Cantor","James J. Collins"],"boundary":"Behavior in one bacterial construct and environment does not establish stability, safety, or equivalent function in other hosts and contexts.","publisher":"Nature","establishes":"The study constructs a mutually repressive bistable gene circuit in Escherichia coli and reports switching under specified inducer and temperature conditions.","identifiers":[{"value":"10.1038/35002131","scheme":"doi"}],"publishedAt":"2000-01-20","exactLocator":"Abstract; Figures 1–6; mathematical model and experimental switching results."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_7904c6b5fd6b46d88a0090495d83ca5f","rationale":"Genetic toggle switch binds claim urn:maha:claim:genetic-toggle-switch only to Construction of a genetic toggle switch in Escherichia coli at Nature version-of-record page, Abstract lines 34–37. The audit inspected version-of-record at declared inspected location depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Construction of a genetic toggle switch in Escherichia coli.”. This decision applies only to record urn:maha:record:genetic-toggle-switch at sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.769Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_736d6bf15173458d8622ae997bf41993","rationale":"Genetic toggle switch remains within domain synthetic-biology. Its mechanism or method is the bounded proposition “The cited study constructs a bistable toggle in Escherichia coli and reports switching thresholds and state behavior under defined induction conditions.”; the record does not transfer that proposition beyond The circuit’s memory and stability cannot be transferred to other hosts, copy numbers, growth conditions, burdens, or timescales. This decision applies only to record urn:maha:record:genetic-toggle-switch at sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.869Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_530edb978fa64bc5bced92ce7ecbf8e9","rationale":"Genetic toggle switch retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:genetic-toggle-switch at sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.943Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"rights-and-locator","verdict":"approve","reviewId":"epireview_71c497f2d33a428e8b937ddbe00d2842","rationale":"Construction of a genetic toggle switch in Escherichia coli is identified by doi:10.1038/35002131, inspected at Abstract; Figures 1–6; mathematical model and experimental switching results., and retained under citation-with-paraphrase. This approval binds only exact revision sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5. This decision applies only to record urn:maha:record:genetic-toggle-switch at sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:05.025Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:5ebe87f4d5c1c2512ae655016d283646d46c04b95f0c8ec643c80f4c847397e5","supersedesReviewId":null,"reviewerProfileVersion":1}]}