{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:repressilator-gene-oscillator","canonicalPath":"/knowledge/synthetic-biology/concepts/repressilator-gene-oscillator","contentHash":"sha256:67bd70ae51f2139f2dbacda76c3e0f42ea9585869a2717136a7d5a1305bcc748","generatedAt":"2026-08-30T16:03:07.830Z","publicationDecision":{"recordId":"urn:maha:record:repressilator-gene-oscillator","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:repressilator-gene-oscillator","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A synthetic oscillatory network of transcriptional regulators.","boundary":"Observed oscillation period, regularity, burden, and persistence are tied to the construct, reporter, host, and environment.","claimKind":"empirical-claim","sourceIds":["source-elowitz-repressilator-2000"],"statement":"The cited study constructs a three-repressor oscillator in Escherichia coli and observes oscillatory fluorescent reporter behavior.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-elowitz-repressilator-2000","url":"https://www.nature.com/articles/35002125","title":"A synthetic oscillatory network of transcriptional regulators","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Michael B. Elowitz","Stanislas Leibler"],"boundary":"The original oscillator’s dynamics are context-specific and do not establish robust timing across hosts, loads, environments, or scales.","publisher":"Nature","establishes":"The study constructs a three-repressor oscillatory network in Escherichia coli and reports oscillatory fluorescence behavior under specified constructs and conditions.","identifiers":[{"value":"10.1038/35002125","scheme":"doi"}],"publishedAt":"2000-01-20","exactLocator":"Abstract; Figures 1–5; model and single-cell fluorescence experiments."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_d400e8ab9cac4cd79c1da56e07b2cf5e","rationale":"Repressilator gene oscillator binds claim urn:maha:claim:repressilator-gene-oscillator only to A synthetic oscillatory network of transcriptional regulators at Nature version-of-record page, Abstract lines 33–36. The audit inspected version-of-record at declared inspected location depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A synthetic oscillatory network of transcriptional regulators.”. This decision applies only to record urn:maha:record:repressilator-gene-oscillator at sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:05.089Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_a289a59e6d014464a042470926c4e9a7","rationale":"Repressilator gene oscillator remains within domain synthetic-biology. Its mechanism or method is the bounded proposition “The cited study constructs a three-repressor oscillator in Escherichia coli and observes oscillatory fluorescent reporter behavior.”; the record does not transfer that proposition beyond Observed oscillation period, regularity, burden, and persistence are tied to the construct, reporter, host, and environment. This decision applies only to record urn:maha:record:repressilator-gene-oscillator at sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:05.163Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_b854f2aac9ad40bbbf7f6203b71ffe98","rationale":"Repressilator gene oscillator retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:repressilator-gene-oscillator at sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:05.223Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"rights-and-locator","verdict":"approve","reviewId":"epireview_47fd9571a41f422e906ba1c8d52c5425","rationale":"A synthetic oscillatory network of transcriptional regulators is identified by doi:10.1038/35002125, inspected at Abstract; Figures 1–5; model and single-cell fluorescence experiments., and retained under citation-with-paraphrase. This approval binds only exact revision sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c. This decision applies only to record urn:maha:record:repressilator-gene-oscillator at sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:05.318Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:4836a066446356da13adb2438a1cf1a694ef61a662b109ea4a7533cf40f04b1c","supersedesReviewId":null,"reviewerProfileVersion":1}]}