{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:single-cell-perturbation-readout","canonicalPath":"/knowledge/synthetic-biology/measurements/single-cell-perturbation-readout","contentHash":"sha256:b9e71532d4dbfcd9ca6f0a343a4bbfb2714fffa715c8f0ddda5eb37b75afeeee","generatedAt":"2026-08-30T16:03:03.347Z","publicationDecision":{"recordId":"urn:maha:record:single-cell-perturbation-readout","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:single-cell-perturbation-readout","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens.","boundary":"Transcriptional state changes are assay- and model-dependent and do not alone prove direct mechanism or organism phenotype.","claimKind":"empirical-claim","sourceIds":["source-dixit-perturb-seq-2016"],"statement":"The cited Perturb-seq study connects CRISPR perturbations with single-cell RNA profiles in specified immune-cell experiments.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-dixit-perturb-seq-2016","url":"https://www.cell.com/cell/fulltext/S0092-8674(16)31610-5","title":"Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Atray Dixit","Oren Parnas","Biyu Li","Jenny Chen","et al."],"boundary":"Transcriptomic association does not by itself establish direct mechanism, organism phenotype, safety, or therapeutic effect.","publisher":"Cell","establishes":"The study combines pooled CRISPR perturbations with single-cell RNA sequencing to resolve perturbation-associated transcriptional states in specified immune-cell experiments.","identifiers":[{"value":"10.1016/j.cell.2016.11.038","scheme":"doi"}],"publishedAt":"2016-12-15","exactLocator":"Summary; Figures 1–7; STAR Methods; supplementary tables and sequencing data."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_ab69b899b621498f8862f16861a4a7f6","rationale":"Single-cell perturbation readout binds claim urn:maha:claim:single-cell-perturbation-readout only to Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens at PMC5181115 author manuscript, Summary and “Perturb-seq: pooled, combinatorial CRISPR screens with scRNA-seq readout”. The audit inspected repository-copy at bounded inspected artifact depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens.”. This decision applies only to record urn:maha:record:single-cell-perturbation-readout at sha256:6425d7ab23e2f018b1b857acf0ece2c747053eaab5bfe0e32810efbffb8626cc and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.256Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:single-cell-perturbation-readout at sha256:6425d7ab23e2f018b1b857acf0ece2c747053eaab5bfe0e32810efbffb8626cc and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.361Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:6425d7ab23e2f018b1b857acf0ece2c747053eaab5bfe0e32810efbffb8626cc","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_87214bcfd1204645b53da41df1f87265","rationale":"Single-cell perturbation readout retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:single-cell-perturbation-readout at sha256:6425d7ab23e2f018b1b857acf0ece2c747053eaab5bfe0e32810efbffb8626cc and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.613Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:single-cell-perturbation-readout at sha256:6425d7ab23e2f018b1b857acf0ece2c747053eaab5bfe0e32810efbffb8626cc and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.696Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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