{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:double-strand-break-repair-outcomes","canonicalPath":"/knowledge/synthetic-biology/mechanisms/double-strand-break-repair-outcomes","contentHash":"sha256:3fdd54c8ac0ec168578662afd045fa07d499ed202d191e2f7fdcb1321715b7bf","generatedAt":"2026-08-30T16:02:39.685Z","publicationDecision":{"recordId":"urn:maha:record:double-strand-break-repair-outcomes","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:double-strand-break-repair-outcomes","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Multiplex Genome Engineering Using CRISPR/Cas Systems.","boundary":"Repair distributions vary with cell state, locus, template, timing, delivery, assay, and selection and cannot be reduced to one universal outcome.","claimKind":"empirical-claim","sourceIds":["source-cong-multiplex-editing-2013"],"statement":"The cited mammalian-cell experiments report locus-specific indels and templated edits after CRISPR-Cas9 cleavage under defined conditions.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-cong-multiplex-editing-2013","url":"https://www.science.org/doi/10.1126/science.1231143","title":"Multiplex Genome Engineering Using CRISPR/Cas Systems","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Le Cong","F. Ann Ran","David Cox","Shuailiang Lin","et al."],"boundary":"Cell-culture demonstrations do not establish organism-level delivery, clinical safety, durable benefit, or universal target performance.","publisher":"Science","establishes":"The study demonstrates CRISPR-Cas9 genome targeting and multiplex editing in mammalian cells under specified constructs, loci, delivery, and assays.","identifiers":[{"value":"10.1126/science.1231143","scheme":"doi"}],"publishedAt":"2013-01-03","exactLocator":"Abstract; Figures 1–4; supplementary methods and target-level results.","conflictsOfInterest":"The publisher record reports patent and company relationships involving CRISPR technologies."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_552d25ae015a462ca509bfbc87da44bd","rationale":"Double-strand-break repair outcomes binds claim urn:maha:claim:double-strand-break-repair-outcomes only to Multiplex Genome Engineering Using CRISPR/Cas Systems at PMC3795411 author manuscript, “Applications of Cas9 for homologous recombination and multiplex genome engineering”. The audit inspected repository-copy at bounded inspected artifact depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Multiplex Genome Engineering Using CRISPR/Cas Systems.”. This decision applies only to record urn:maha:record:double-strand-break-repair-outcomes at sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:01:59.272Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_aaab0630e9414ed98cc54b82fabba2fd","rationale":"Double-strand-break repair outcomes remains within domain synthetic-biology. Its mechanism or method is the bounded proposition “The cited mammalian-cell experiments report locus-specific indels and templated edits after CRISPR-Cas9 cleavage under defined conditions.”; the record does not transfer that proposition beyond Repair distributions vary with cell state, locus, template, timing, delivery, assay, and selection and cannot be reduced to one universal outcome. This decision applies only to record urn:maha:record:double-strand-break-repair-outcomes at sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:01:59.373Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_f52f7f370efb4af48f09cc1c8300b2bb","rationale":"Double-strand-break repair outcomes retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:double-strand-break-repair-outcomes at sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:01:59.445Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"rights-and-locator","verdict":"approve","reviewId":"epireview_8ed1432af6e142fc96447f78fa0dce3d","rationale":"Multiplex Genome Engineering Using CRISPR/Cas Systems is identified by doi:10.1126/science.1231143, inspected at Abstract; Figures 1–4; supplementary methods and target-level results., and retained under citation-with-paraphrase. This approval binds only exact revision sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660. This decision applies only to record urn:maha:record:double-strand-break-repair-outcomes at sha256:6560279b2afb7f1850f4ee8877679effb88c69af535aa3669b6410ebd5348660 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:01:59.523Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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