{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:prime-editing-guide-rna-mechanism","canonicalPath":"/knowledge/synthetic-biology/mechanisms/prime-editing-guide-rna-mechanism","contentHash":"sha256:566c8d24c285a007c6b350bde56eb4110e4766cda65e2d2de29a81796482f103","generatedAt":"2026-08-30T16:02:43.648Z","publicationDecision":{"recordId":"urn:maha:record:prime-editing-guide-rna-mechanism","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:prime-editing-guide-rna-mechanism","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Search-and-replace genome editing without double-strand breaks or donor DNA.","boundary":"A pegRNA design does not predict universal efficiency, purity, delivery, or organism-level consequence.","claimKind":"empirical-claim","sourceIds":["source-anzalone-prime-2019-graph"],"statement":"The cited study demonstrates that pegRNA sequence elements and a nickase–reverse-transcriptase fusion jointly specify intended edit outcomes in named cell systems.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-anzalone-prime-2019-graph","url":"https://www.nature.com/articles/s41586-019-1711-4","title":"Search-and-replace genome editing without double-strand breaks or donor DNA","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Andrew V. Anzalone","Peyton B. Randolph","Jessie R. Davis","Alexander A. Sousa","et al."],"boundary":"The study does not establish general delivery, organism-level safety, clinical benefit, or universal editing efficiency.","publisher":"Nature","establishes":"The study introduces a Cas9 nickase–reverse-transcriptase editor and prime-editing guide RNA architecture and reports specified edit classes in named cell systems.","identifiers":[{"value":"10.1038/s41586-019-1711-4","scheme":"doi"}],"publishedAt":"2019-10-21","exactLocator":"Abstract; Figures 1–5; Methods; Extended Data; Supplementary Tables 1–5.","conflictsOfInterest":"The article declares patent applications and company relationships involving genome editing."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_306938f1c5c3489ba31509ae5a080e21","rationale":"Prime-editing guide RNA mechanism binds claim urn:maha:claim:prime-editing-guide-rna-mechanism only to Search-and-replace genome editing without double-strand breaks or donor DNA at PMC6907074 author manuscript, Figure 1 and “Prime editing strategy”. The audit inspected repository-copy at bounded inspected artifact depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Search-and-replace genome editing without double-strand breaks or donor DNA.”. This decision applies only to record urn:maha:record:prime-editing-guide-rna-mechanism at sha256:0b3e9a4522c5b0be9b6ce514fe55945920acf5364ab9c63701ab177042d8ce10 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:00.353Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:prime-editing-guide-rna-mechanism at sha256:0b3e9a4522c5b0be9b6ce514fe55945920acf5364ab9c63701ab177042d8ce10 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:00.435Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:prime-editing-guide-rna-mechanism at sha256:0b3e9a4522c5b0be9b6ce514fe55945920acf5364ab9c63701ab177042d8ce10 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:00.524Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:prime-editing-guide-rna-mechanism at sha256:0b3e9a4522c5b0be9b6ce514fe55945920acf5364ab9c63701ab177042d8ce10 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:00.605Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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