{"schemaVersion":"maha-epistemic/1.0","evidencePolicyVersion":"mps/0.1","recordId":"urn:maha:record:pooled-crispr-screening","canonicalPath":"/knowledge/synthetic-biology/methods/pooled-crispr-screening","contentHash":"sha256:b5b4f2fe8862b93d6f65e0a66a98e8611e52e04c4bfe15981c2682714cdcda56","generatedAt":"2026-08-30T16:03:00.764Z","publicationDecision":{"recordId":"urn:maha:record:pooled-crispr-screening","publicEligible":true,"evaluatedAgainst":"maha-epistemic/1.0","reasons":[]},"claims":[{"id":"urn:maha:claim:pooled-crispr-screening","scope":"The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.","boundary":"Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects.","claimKind":"empirical-claim","sourceIds":["source-shalem-crispr-screen-2014"],"statement":"The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models.","replication":{"asOfDate":"2026-08-24","assessment":"This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.","independentReplicationCount":null},"uncertainty":{"kind":"qualitative","statement":"There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices."},"evidenceMaturity":"single-study"}],"sources":[{"id":"source-shalem-crispr-screen-2014","url":"https://www.science.org/doi/10.1126/science.1247005","title":"Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells","rights":{"note":"Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.","basis":"citation-with-paraphrase","quotationUsed":false},"authors":["Ophir Shalem","Neville E. Sanjana","Ella Hartenian","Xi Shi","et al."],"boundary":"Screen enrichment is model-, library-, delivery-, coverage-, phenotype-, and analysis-specific and is not direct clinical or organism-level evidence.","publisher":"Science","establishes":"The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models.","identifiers":[{"value":"10.1126/science.1247005","scheme":"doi"}],"publishedAt":"2013-12-12","exactLocator":"Abstract; Figures 1–4; supplementary methods; library and screen-analysis sections."}],"reviewEvents":[{"scope":"source-fidelity","verdict":"approve","reviewId":"epireview_ca1ddeb0a1df4ea9a37599af3e62d11f","rationale":"Pooled CRISPR screening binds claim urn:maha:claim:pooled-crispr-screening only to Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells at PMC4089965 author manuscript, Abstract and pooled GeCKO negative- and positive-selection sections. The audit inspected repository-copy at bounded inspected artifact depth and recorded subject and claim support; the claim remains limited to “The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.”. This decision applies only to record urn:maha:record:pooled-crispr-screening at sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:03.961Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"domain-fidelity","verdict":"approve","reviewId":"epireview_a2c6581535d144319de2242da2164291","rationale":"Pooled CRISPR screening remains within domain synthetic-biology. Its mechanism or method is the bounded proposition “The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models.”; the record does not transfer that proposition beyond Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects. This decision applies only to record urn:maha:record:pooled-crispr-screening at sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.030Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7","supersedesReviewId":null,"reviewerProfileVersion":1},{"scope":"boundary-adequacy","verdict":"approve","reviewId":"epireview_0f01c01108064ec0bf144d0555e4d1c0","rationale":"Pooled CRISPR screening retains uncertainty “There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.” and replication assessment “This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.”. Its non-claims and 2 prohibited inference(s) remain attached to every reuse. This decision applies only to record urn:maha:record:pooled-crispr-screening at sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.116Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. 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This decision applies only to record urn:maha:record:pooled-crispr-screening at sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7 and does not certify truth, external endorsement, independent reproduction, or fitness for use.","reviewedAt":"2026-08-30T16:02:04.188Z","reviewerId":"expert_maha-internal-editorial-scale-v1","reviewMethod":"Each criterion is recomputed from the exact record, its inspected alignment audit, source identity, exact locator, rights basis, claim scope, boundary, uncertainty, replication status, prohibited inferences, and revision digest.","reviewerKind":"internal-editorial","reviewerRole":"AI-assisted record-specific review of inspected source identity, exact locator, bounded claim, uncertainty, non-claims, rights basis, and exact revision. This is not an external subject-matter credential.","targetSha256":"sha256:959e5b4a8a42988da114a0702a7a9d34381c190305a42ae647f6e061d1f8f2c7","supersedesReviewId":null,"reviewerProfileVersion":1}]}