Bounded definition
A distinction between assays that identify candidate sites and cellular measurements that confirm editing frequency under matched conditions.
What the cited work establishes
The study develops an in-vitro circularized-DNA assay for Cas9 activity and compares nominations with cellular activity and genomic context for specified targets.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity.
Claims: urn:maha:claim:off-target-nomination-versus-confirmation
What remains a separate question
A nominated site is not automatically edited in cells, while a non-nominated site is not proof of absolute absence below every detection limit.
In-vitro nomination is not identical to editing frequency or biological consequence in a treated cell population or organism.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
GUIDE-seq off-target detection
inbound connection · method
GUIDE-seq produces a nomination set that requires orthogonal confirmation and frequency measurement.
CHANGE-seq off-target nomination
inbound connection · method
CHANGE-seq nominations require cellular confirmation under matched editor and delivery conditions.
GUIDE-seq off-target detection
outbound connection · method
GUIDE-seq provides a cell-based nomination channel.
CHANGE-seq off-target nomination
outbound connection · method
CHANGE-seq provides an in-vitro nomination channel.
Claim ledger
Every proposition keeps its own evidence state.
The cited study compares in-vitro CHANGE-seq nominations with cellular off-target activity and shows that genomic context and individual variation affect correspondence.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity.
- Boundary
- A nominated site is not automatically edited in cells, while a non-nominated site is not proof of absolute absence below every detection limit.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Nature Biotechnology
CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
Christopher R. Lazzarotto, Nhu T. Nguyen, J. A. Tangprasertchai, S. C. Malagon-Lopez, et al.
- Exact locator
- Abstract; Figures 1–6; Methods; datasets PRJNA625995 and GSE149295.
- Establishes
- The study develops an in-vitro circularized-DNA assay for Cas9 activity and compares nominations with cellular activity and genomic context for specified targets.
- Boundary
- In-vitro nomination is not identical to editing frequency or biological consequence in a treated cell population or organism.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.
- Declared interests
- The article declares patent and company relationships involving genome-editing assays and therapeutics.