Bounded definition
A cell-based sequencing method that captures double-strand oligonucleotides at nuclease-induced DNA breaks to nominate off-target sites.
What the cited work establishes
The study develops GUIDE-seq, a cell-based double-strand-break capture method, and applies it to specified nucleases and human cell lines.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.
Claims: urn:maha:claim:guide-seq-off-target-detection
What remains a separate question
Assay sensitivity depends on break capture, cell compatibility, sequencing, analysis, and the nuclease modality under study.
GUIDE-seq has assay-specific detection limits and dependencies and does not nominate every possible off-target event in every biological context.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Off-target nomination versus confirmation
outbound connection · comparison
GUIDE-seq produces a nomination set that requires orthogonal confirmation and frequency measurement.
Off-target nomination versus confirmation
inbound connection · comparison
GUIDE-seq provides a cell-based nomination channel.
Claim ledger
Every proposition keeps its own evidence state.
The cited study develops GUIDE-seq and applies it to specified RNA-guided nucleases in two human cell lines.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.
- Boundary
- Assay sensitivity depends on break capture, cell compatibility, sequencing, analysis, and the nuclease modality under study.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Nature Biotechnology
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Shengdar Q. Tsai, Zongli Zheng, Nhu T. Nguyen, Matthew Liebers, et al.
- Exact locator
- Abstract; Figures 1–6; Online Methods; Supplementary Tables; Sequence Read Archive SRP050338.
- Establishes
- The study develops GUIDE-seq, a cell-based double-strand-break capture method, and applies it to specified nucleases and human cell lines.
- Boundary
- GUIDE-seq has assay-specific detection limits and dependencies and does not nominate every possible off-target event in every biological context.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.