Bounded definition
Locus-focused sequencing used to quantify intended edits, indels, and selected nominated off-target outcomes.
What the cited work establishes
The study develops GUIDE-seq, a cell-based double-strand-break capture method, and applies it to specified nucleases and human cell lines.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.
Claims: urn:maha:claim:targeted-amplicon-sequencing
What remains a separate question
Amplicon assays are limited by primer design, sampled sites, allele representation, read depth, error correction, and variant-calling thresholds.
GUIDE-seq has assay-specific detection limits and dependencies and does not nominate every possible off-target event in every biological context.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Editing efficiency and byproduct measurement
outbound connection · measurement
Targeted sequencing supplies one measurement channel for intended products and local byproducts.
Editing efficiency and byproduct measurement
inbound connection · measurement
Targeted sequencing is one method used to resolve the product distribution.
Claim ledger
Every proposition keeps its own evidence state.
The cited GUIDE-seq workflow uses targeted sequencing and validation to characterize nominated cleavage sites under specified experiments.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.
- Boundary
- Amplicon assays are limited by primer design, sampled sites, allele representation, read depth, error correction, and variant-calling thresholds.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Nature Biotechnology
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
Shengdar Q. Tsai, Zongli Zheng, Nhu T. Nguyen, Matthew Liebers, et al.
- Exact locator
- Abstract; Figures 1–6; Online Methods; Supplementary Tables; Sequence Read Archive SRP050338.
- Establishes
- The study develops GUIDE-seq, a cell-based double-strand-break capture method, and applies it to specified nucleases and human cell lines.
- Boundary
- GUIDE-seq has assay-specific detection limits and dependencies and does not nominate every possible off-target event in every biological context.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.