Bounded definition
A boundary between edits confined to treated somatic cells and edits made in embryos or reproductive-line cells that may become heritable.
What the cited work establishes
The study reports CRISPR-Cas9 editing outcomes, mosaicism, and unintended events in non-viable tripronuclear human zygotes under the stated protocol.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes.
Claims: urn:maha:claim:somatic-versus-germline-editing
What remains a separate question
The experiment does not establish safe or acceptable reproductive use; germline questions require distinct ethical, legal, clinical, and multigenerational evidence.
The experiment does not establish reproductive safety, heritable clinical benefit, ethical acceptability, or authorization for germline use.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Ex-vivo genome-editing workflow
inbound connection · method
The reported ex-vivo intervention edits somatic hematopoietic cells rather than reproductive-line cells.
Ex-vivo genome-editing workflow
outbound connection · method
The cited ex-vivo hematopoietic workflow targets somatic cells and is not a germline intervention.
Claim ledger
Every proposition keeps its own evidence state.
The cited embryo study reports mosaic and unintended outcomes after CRISPR-Cas9 exposure of non-viable tripronuclear human zygotes.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes.
- Boundary
- The experiment does not establish safe or acceptable reproductive use; germline questions require distinct ethical, legal, clinical, and multigenerational evidence.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Protein & Cell
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
Puping Liang, Yanwen Xu, Xiya Zhang, Chenhui Ding, et al.
- Exact locator
- Abstract; Results; Tables 1–2; Methods; ethical statement.
- Establishes
- The study reports CRISPR-Cas9 editing outcomes, mosaicism, and unintended events in non-viable tripronuclear human zygotes under the stated protocol.
- Boundary
- The experiment does not establish reproductive safety, heritable clinical benefit, ethical acceptability, or authorization for germline use.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.