Bounded definition
A Cas-guided deaminase architecture that can install selected C-to-T outcomes without a programmed double-strand break.
What the cited work establishes
The study constructs cytosine base editors and reports targeted C-to-T conversion outcomes in specified transformed human and murine cell lines.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
Claims: urn:maha:claim:cytosine-base-editing
What remains a separate question
Reported activity windows, product purity, bystanders, indels, and off-target effects are editor- and context-specific.
The reported windows, efficiencies, byproducts, and indels are editor-, guide-, target-, cell-, delivery-, and assay-specific.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Editing efficiency and byproduct measurement
outbound connection · measurement
Base-editing claims require joint measurement of intended products and byproducts.
Claim ledger
Every proposition keeps its own evidence state.
The cited study constructs and tests cytosine base editors in specified transformed human and murine cell lines.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
- Boundary
- Reported activity windows, product purity, bystanders, indels, and off-target effects are editor- and context-specific.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Nature
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor, Yongjoo B. Kim, Michael S. Packer, John A. Zuris, David R. Liu
- Exact locator
- Abstract; Figures 1–5; Methods; Extended Data; Supplementary Information.
- Establishes
- The study constructs cytosine base editors and reports targeted C-to-T conversion outcomes in specified transformed human and murine cell lines.
- Boundary
- The reported windows, efficiencies, byproducts, and indels are editor-, guide-, target-, cell-, delivery-, and assay-specific.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.
- Declared interests
- The article declares patent applications and company interests involving base-editing technologies.