Bounded definition
The targeting mechanism that combines guide–DNA complementarity with protospacer-adjacent-motif recognition by Cas9.
What the cited work establishes
The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Claims: urn:maha:claim:guide-rna-and-pam-recognition
What remains a separate question
Sequence recognition rules do not by themselves predict chromatin access, cellular activity, or every off-target interaction.
An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
CRISPR-Cas9 nuclease editing
inbound connection · concept
Target recognition depends on guide complementarity and a compatible adjacent motif.
CRISPR-Cas9 nuclease editing
outbound connection · concept
Guide and motif recognition direct the nuclease mechanism.
Claim ledger
Every proposition keeps its own evidence state.
The cited experiments show that guide sequence and adjacent motif requirements condition Cas9 binding and cleavage in the reconstructed system.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
- Boundary
- Sequence recognition rules do not by themselves predict chromatin access, cellular activity, or every off-target interaction.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Science
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier
- Exact locator
- Abstract; Figures 1–5; supplementary materials describing guide design and cleavage assays.
- Establishes
- The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif.
- Boundary
- An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.