Bounded definition
RNA-guided DNA cleavage using a Cas9 nuclease and target-complementary guide sequence.
What the cited work establishes
The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Claims: urn:maha:claim:crispr-cas9-nuclease-editing
What remains a separate question
Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism.
An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Guide RNA and PAM recognition
outbound connection · mechanism
Target recognition depends on guide complementarity and a compatible adjacent motif.
Guide RNA and PAM recognition
inbound connection · mechanism
Guide and motif recognition direct the nuclease mechanism.
Double-strand-break repair outcomes
inbound connection · mechanism
Nuclease editing delegates the final sequence outcome to cellular break repair.
Claim ledger
Every proposition keeps its own evidence state.
The cited biochemical study reconstitutes programmable Cas9 cleavage and shows that a designed single guide can direct sequence-specific cutting adjacent to a compatible motif.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
- Boundary
- Biochemical cleavage is not equivalent to efficient, specific, or safe genome editing in a cell or organism.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Science
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity
Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A. Doudna, Emmanuelle Charpentier
- Exact locator
- Abstract; Figures 1–5; supplementary materials describing guide design and cleavage assays.
- Establishes
- The study reconstitutes RNA-programmed Cas9 cleavage in vitro and shows that a designed single-guide RNA can direct sequence-specific DNA cleavage adjacent to an appropriate motif.
- Boundary
- An in-vitro nuclease mechanism does not establish editing efficiency, specificity, delivery, safety, or phenotype in cells or organisms.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.