Bounded definition
Parallel genetic perturbation using a guide library, selectable phenotype, and sequencing-based guide abundance readout.
What the cited work establishes
The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models.
The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.
Claims: urn:maha:claim:pooled-crispr-screening
What remains a separate question
Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects.
Screen enrichment is model-, library-, delivery-, coverage-, phenotype-, and analysis-specific and is not direct clinical or organism-level evidence.
Connected domain graph
Typed dependencies preserve publication state.
Only independently canonical records receive public links and relation statements. Draft graph topology remains private.
Single-cell perturbation readout
outbound connection · measurement
Single-cell readouts add state-resolved measurements to pooled perturbation designs.
Single-cell perturbation readout
inbound connection · measurement
Perturb-seq extends pooled screens with cell-resolved transcriptomic outcomes.
Claim ledger
Every proposition keeps its own evidence state.
The cited study develops genome-scale CRISPR knockout libraries and reports positive and negative selection screens in specified human cell models.
- Scope
- The constructs, biological systems, protocols, assays, datasets, and comparisons reported in Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells.
- Boundary
- Guide enrichment identifies screen-dependent associations and can reflect editing, growth, delivery, copy number, sampling, and model-specific effects.
- Uncertainty
- There is no universal effect estimate for this method; numerical results remain attached to the source experiment, biological system, assay, and analysis choices.
- Replication
- This candidate records one bounded source package. Independent replications and contradictory results must be compiled separately before evidence maturity is upgraded.
Primary sources
Citation, locator, rights, and boundary travel together.
Source 1 · Science
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi, et al.
- Exact locator
- Abstract; Figures 1–4; supplementary methods; library and screen-analysis sections.
- Establishes
- The study develops a pooled lentiviral CRISPR knockout library and applies positive and negative selection screens in specified human cell models.
- Boundary
- Screen enrichment is model-, library-, delivery-, coverage-, phenotype-, and analysis-specific and is not direct clinical or organism-level evidence.
- Rights basis
- citation with paraphrase · Maha paraphrases the source-level result and links to the version of record; no article passage is reproduced.